Heterotopic Human Breast Ductal Carcinoma Model in Athymic Nude Mouse

Overview

• Human Breast Cell Line BT-474
• Cultured cells injected into subcutaneous compartment of nude (athymic) mice
• Mice must be pretreated with 0.72mg/day 17 beta-estradiol implant
• Readouts: Tumor size by caliper
• Histopathology of major organ system

Timeline:

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Protocol:

• Athymic nu female mice (90+ days)
• Groups Size: n=10
• Pre-treatment:
  • Estrogen Implant (Day -1, 0.72mg pellet/mouse)
  • BT-474 cells inoculated (Day 1, 10⁷ cells, SC)
• Randomization (Day 10-14):
  • Tumor Size (ranging from 0.2-0.3 cm³
  • Body Weight
• Treatment (Day 14-49):
  • Tumor Size (ranging from 0.2-0.3 cm³
  • Body Weight
• Analyses:
  • Clinical Observations: health, tumor size, body weight
  • Gross and Histopathology