Animal Models of Inflammatory Disease

Rectal administration of 2,4 6-trinitrobenzenesulfonic acid
Model for inflammatory bowel disease composed of ulcerative colitis and Crohn's disease
IBD results in chronic inflammation of bowel, ulcerogenesis and diarrhea
Currently few treatment options
(TNBS) is well established model of IBD
Can be performed in rats or mice

In life observations

Gross pathological changes in colon
Clinical Scoring of Colon, Measured day 7 and day 14 post induction Colons present with damage at day 7

Treatment	Colon Wt (g)	Colon Score (d7)
Saline	1.02	0
EtOH	1.5	1.2
EtOH+TNBS	3.8	4
0 – No damage 1 – Localized hyperemia, no damage 2 – Linear ulceration with inflammation at 1 site 3 – Linear ulceration with inflammation at 2 or more sites 4 – Two or more major sites of inflammation and bulcerations, or one major site extending >1cm along the colon

Histological changes in colon

Total IBD Index Score